Product Information | |
Cat#No# | AC-027A |
Common Name | Protein L |
Product Overview | rProtein L Agarose 4 Fast Flow is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, as well as Fab, scFv fragments of immunoglobulins from biological fluids and from cell culture media at both laboratory and process scale. Protein L is first isolated from the surface of bacterial species Peptostreptoccus magnus and now recombinant Protein L is obtained by gene recombination technology. Unlike Protein A and Protein G, which bind primarily through Fc regions of immunoglobulins, Protein L binds the variable region of antibodies kappa light chain and binds a wider range of Ig classes and subclasses. Protein L is therefore particular suitable for capture of a wide range of different-sized antibody fragments such as Fab fragments, single-chain variable fragments (scFv) and domain antibodies (dAbs). It is also suitable for mouse IgM purification from cell. rProtein L Agarose 4 Fast Flow utilizes a stable thioether linkage between rProtein L and the cross-linked agarose beads and is optimized for high binding capacity for immunoglobulins. |
Matrix | Cross-linked 4% agarose supplied as a 50% slurry |
Average particle size | 45 μm - 165 μm |
Ligand | Recombinant protein L produced in E.coli |
Dynamic binding capacity | >15 mg human IgG/mL medium |
Recommended flow rate | Gravity flow and medium pressure, 30 - 300 cm/hour |
Maximum Pressure Drop | 0.3 MPa (3 bar) |
Chemical stability | All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea. |
Storage | 2 - 8°C, storage buffer: 20% ethanol |
Download Datasheet |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Online Inquiry