rProtein L Agarose Resin
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rProtein L Agarose Resin

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AC-012A rProtein L Agarose Resin 5ml Inquiry
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Product Information
Cat#No# AC-012A
Common Name Protein L
Product Overview rProtein L Agarose is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, as well as Fab, scFv fragments of immunoglobulins from biological fluids and from cell culture media at both laboratory and process scale.

Protein L is first isolated from the surface of bacterial species Peptostreptoccus magnus and now recombinant Protein L is obtained by gene recombination technology. Unlike Protein A and Protein G, which bind primarily through Fc regions of immunoglobulins, Protein L binds the variable region of antibodies kappa light chain and binds a wider range of Ig classes and subclasses. Protein L is therefore particular suitable for capture of a wide range of different-sized antibody fragments such as Fab fragments, single-chain variable fragments (scFv) and domain antibodies (dAbs). It is also suitable for mouse IgM purification from the cell.
Matrix 4% agarose supplied as a 50% slurry
Average particle size 45 μm - 165 μm
Ligand Recombinant protein L produced in E.coli
Dynamic binding capacity >15 mg human IgG/mL medium
Recommended flow rate Gravity flow and medium pressure, 30 - 300 cm/hour
Maximum Pressure Drop 0.1 MPa (1 bar)
Chemical stability All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.
Storage 2 - 8°C, storage buffer: 20% ethanol
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For Research or Industrial Raw Materials, Not For Personal Medical Use!
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