rTrypsin 3.0 Protease, MS Grade

• sizes: 20 μg
• Description: rTrypsin 3.0 is a recombinant protease that specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Engineered with a redesigned gene sequence and methylated lysines, it offers ultra-fast protein hydrolysis, high activity, and no self-degradation. rTrypsin 3.0 is ideal for protein characterization, single-cell proteomics, and large cohort proteomics studies.
• Physical Appearance: Lyophilized powder with 20 μg Trehalose
• Molecular Weight: 23.7 kDa
• Resuspension Buffer: Dissolve in 40 μL of 50 mM acetic acid to a concentration of 0.5 μg/μL
• Storage: Store the lyophilized powder at –20 °C; store reconstituted enzyme at –80 °C
• Shelf life: 24 months at -20 ℃
• Stability: rTrypsin 3.0 is maximally active in the pH range of 7–9
• Usage Notes: 1. Suitable enzyme digestion buffers: 20 mM Tris, 50 mM ABC, or HEPES buffer; pH 7-9. 2. Use a 1:50 protease-to-protein ratio with a protein concentration of 0.5 µg/µL. Incubate at 37°C in a dry bath for 30 minutes.
• Activity: rTrypsin: 15,000 units/mg.
• Unit Definition: At pH 7.8 and 25°C, 1.0 μmol of BAEE is hydrolyzed per minute (detected at 253 nm).