rTrypsin/rLys C Mixer Protease, MS Grade

• sizes: 20 μg
• Description: The rTrypsin 3.0 and rLys C enzyme mix combines two recombinant proteases for efficient peptide bond hydrolysis. rTrypsin 3.0 cleaves at the carboxyl side of lysine and arginine residues, while rLys C targets lysine residues specifically. This combination minimizes missed cleavages caused by rTrypsin’s slower digestion of lysine and arginine, PTMs on lysines, or hydrophobic C-termini (e.g., proline). The rTrypsin and rLys C mix is ideal for protein characterization, single-cell proteomics, and large cohort proteomics studies.
• Physical Appearance: Lyophilized powder with 67 µg Trehalose
• Molecular Weight: rTrypsin 23.7 kDa; rLys C 27 kDa
• Resuspension Buffer: Dissolve in 40 µL of 50 mM acetic acid to a concentration of 0.5 µg/µL
• Storage: Lyophilized powder: –20°C; Reconstituted enzyme: –80°C
• Shelf life: 24 months at -20 ℃
• Stability: rTrypsin 3.0/rLys C is maximally active in the pH range of 7–9
• Usage Notes: 1. Suitable digestion buffers: 20 mM Tris, 50 mM ABC, or HEPES buffer (pH 7–9). 2. Use denatured protein samples at a 1:50 protease-to-protein ratio. Maintain a protein concentration of 0.5 µg/µL and incubate at 37°C for 30 minutes.
• Activity: • rTrypsin: 15,000 units/mg. • rLys C: 25 units/mg.
• Unit Definition: 1.0 µmol BAEE hydrolyzed per minute at pH 7.8, 25°C, 253 nm. 1.0 µmol T-G-P Lys Nitroanilide hydrolyzed per minute at pH 7.8, 25°C, 405 nm.