Sephacryl S-100 HR

• Cat#No#: Se-475C
• Product Overview: Sephacryl High Resolution size exclusion chromatography resins allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by size exclusion chromatography at laboratory and industrial scale.
• Description: Sephacryl High Resolution chromatography resins is a highly versatile size exclusion chromatography resins that offers a wide range of fractionation capabilities. The matrix of Sephacryl High Resolution resins is a cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide. This crosslinking gives good rigidity and chemical stability. The narrow particle size distribution, together with steep selectivity curves, results in good preparative characteristics with maintained high resolution. The hydrophilic nature of the resins minimizes nonspecific adsorption and maximizes recovery. The excellent resolution and flow characteristics, long-term physical and chemical stability, and ease of handling make Sephacryl HR the resin of choice for routine purification.
• Characteristic: Cost-efficient size exclusion chromatography resin providing high resolution with fast and easy operation.
  Five different fractionation ranges available in Sephacryl HR family allows for reproducible separations over a wide molecular weight range.
  Sephacryl S-100 HR is designed for separating peptides and small proteins (fractionation range globular proteins 1 × 10– 1 × 10⁵). BioProcess resin supported for industrial applications and well-established in approved processes.
• Maximum operating pressure: ≥125 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20°C using buffers with a viscosity similar to water).
• Matrix: Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.
• Average particle size: ~50 µm
• Recommended column height: 15 cm
• Chemical stability: Commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile, 0.5 M NaOH (only for cleaning-in-place), 2 M NaCl.
• pH working range: 3–11
• CIP stability: 2–13
• Autoclavable: 20 min at 121°C in 0.15 M NaCl pH 7, 5 cycles.
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: Wash the resin in the column with 0.1 M NaOH at 10 cm/h with reversed flow direction. Contact time 1 h.
• Pack size: 750 mL
• BioProcess resin: Yes
• Dimensions: 5 cm