Sephacryl S-300 HR

• Cat#No#: Se-477C
• Product Overview: Sephacryl High Resolution size exclusion chromatography resins allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by size exclusion chromatography at laboratory and industrial scale.
• Description: Sephacryl High Resolution chromatography resins is a highly versatile gel filtration resins that offers a wide range of fractionation capabilities. The matrix of Sephacryl High Resolution resins is a cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide. This crosslinking gives good rigidity and chemical stability. The narrow particle size distribution, together with steep selectivity curves, results in good preparative characteristics with maintained high resolution. The hydrophilic nature of the resins minimizes nonspecific adsorption and maximizes recovery. The excellent resolution and flow characteristics, long-term physical and chemical stability, and ease of handling make Sephacryl HR the resin of choice for routine purification.
• Characteristic: Cost-efficient gel filtration resins providing high resolution with fast and easy operation.
  Five different fractionation ranges available in Sephacryl HR family allows for reproducible separations over a wide molecular weight range.
  Sephacryl S-200 High Resolution resin is designed for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 5 × 10³ – 2.5 × 10⁵).
  BioProcess resin supported for industrial applications and well-established in approved processes.
• Maximum operating pressure: ≥150 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20°C using buffers with a viscosity similar to water).
• Matrix: Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.
• Average particle size: ~50 µm
• Recommended column height: 15 cm
• Chemical stability: Commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile, 0.5 M NaOH (only for cleaning-in-place), 2 M NaCl.
• pH working range: 3–11
• CIP stability: 2–13
• Temperature stability: 4°C to 30°C
• Autoclavable: 20 min at 121°C in 0.15 M NaCl pH 7, 5 cycles.
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: Wash the column with 0.2 to 0.5 M NaOH or a solution of a nonionic detergent at a flow velocity of 15 to 20 cm/h. The total contact time with the cleaning solution must be 1 to 2 h. After washing always re-equilibrate the column with 2 column volumes of buffer.
• Sanitization: Wash the resin in the column with 0.1 M NaOH at 10 cm/h with reversed flow direction. Contact time 1 h.
• Pack size: 750 mL
• BioProcess resin: Yes
• Dimensions: 5 cm