Sephacryl S-300 HR SEC resin in HiPrep Columns

• Cat#No#: Se-318P
• Product Overview: HiPrep Sephacryl S-300 HR columns are designed for routine size exclusion chromatography of proteins, such as antibodies and serum proteins.
• Description: Sephacryl High Resolution (HR) chromatography resins allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by size exclusion chromatography (SEC) at laboratory and industrial scale. Five Sephacryl HR chromatography resins are available as prepacked columns and in laboratory and larger pack sizes: Sephacryl S-100 HR, S-200 HR, S-300 HR, S-400 HR, and S-500 HR.
• Characteristic: Wide fractionation range – Mr 10 000–1 500 000 for globular proteins, well suited for antibody purification.
  High capacity columns – preparative separation of proteins in sample volumes up to 5 mL or 13 mL for 16/60 and 26/60 columns, respectively.
  Convenient – prepacked Sephacryl columns are easily connected to ӒKTA systems for automated chromatography at low to medium pressure.
• Maximum operating pressure: 0.15 MPa (1.5 bar, 21.7 psi)
• Matrix: Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.
• Average particle size: ~ 50 µm
• Recommended flow rate: 15 cm/h
• Recommended column height: 600 mm
• Chemical stability: Stable to commonly used aqueous buffers: 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, 2 M NaCl, 20% ethanol, 30% propanol, 30% acetonitrile.
• Physical stability: Negligible volume variation due to changes in pH or ionic strength.
• pH working range: 3 - 11
• CIP stability: 2 - 13
• Autoclavable: 20 min at 121°C in 0.15 M NaCl pH 7, 5 cycles (resin only).
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: 0.5 M NaOH at 15 cm/h
• Maximum flow velocity: 1.0 mL/min
• Dimensions: 16 × 600 mm
• Column volume: 120 mL
• Column i.d.: 16 mm
• Column hardware pressure limit: 0.5 MPa (5 bar, 72.5 psi)