Sephadex G-10

• Cat#No#: Se-480C
• Product Overview: Sephadex G-10 is a well established gel filtration resin for desalting and buffer exchange of peptides and small biomolecules >700 molecular weight. Smallest exclusion limit in the Sephadex range.
• Description: Sephadex is a gel filtration resin prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, resin, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency fractionation with shorter diffusion distances. Coarse and resin are are preferred for large scale group separations where high flow rates and low operating pressures are required.
• Characteristic: Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  Suitable for buffer exchange and cleanup of biological samples, for example peptides, small proteins, and oligosaccharides larger than 700 molecular weight.
  Efficient removal of contaminants such as salts, dyes, and radioactive labels.
  Lowest exclusion limit (700 molecular weight) in the Sephadex G-range.
• Maximum operating pressure: 24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d
• Matrix: Cross-linked dextran
• Particle Size: wet: 55–165 µm; Dry: 40 µm-120 µm.
• Recommended flow rate: < Darcy´s Law
• Recommended column height: 30 cm
• pH working range: 2–13
• Autoclavable: In wet form (pH 7.0) at 120°C for 30 min.
• Storage: 4 to 30°C
• Pack size: 100 g