Sephadex G-50 Medium

• Cat#No#: Se-490C
• Product Overview: Sephadex G-50 resin is well established gel filtration resin for desalting and buffer exchange of biomolecules >30 000 molecular weight. The larger bead size of G-50 resin is suited for large scale separations.
• Description: Sephadex is a gel filtration resin prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, resin, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.
• Characteristic: Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  Suitable for purification of DNA from small molecules by gel filtration.
  Larger bead size for large scale group separations where high flow rates and low operating pressures are required.
  Classic gel filtration resin.
• Applications: For the separation of molecules Mr >30 000 from molecules Mr <1500 such as labeled protein or DNA from unconjugated dyes For removeing small nucleotides from longer chain nucleic acids.
• Maximum operating pressure: min 150 cm/h, bed height 10 cm, column 5 cm i.d.
• Matrix: Cross-linked dextran
• Particle Size: wet: 100–300 µm; Dry: 50 µm-150 µm.
• Recommended flow rate: < Darcy´s Law
• Recommended column height: 10 cm
• pH working range: 2–10
• CIP stability: 2–13
• Autoclavable: In wet form (pH 7.0) at 120°C for 30 min.
• Storage: 4 to 30°C
• Pack size: 100 g