Sepharose 4B
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Sepharose 4B

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Se-497C Sepharose 4B Inquiry
Product Information
Cat#No# Se-497C
Product Overview Sepharose 4B is a well-proven agarose size exclusion chromatography base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
Description Sepharose is a spherical agarose-based size exclusion chromatography matrix. Sepharose is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose 2B, Sepharose 4B and Sepharose 6B respectively, Both Sepharose and Sepharose CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight.
Characteristic 4% Agarose size exclusion chromatography resin.
Proven base matrix for coupling affinity ligands.
Maximum operating pressure 70-140 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d.
Matrix agarose, 4%, spherical
Particle Size 45 μm-165 μm
Recommended flow rate < 11.5 cm/h
Recommended column height 10 cm
Chemical stability Stable to commonly used aqueous buffers.
Physical stability Negligible volume variation due to changes in pH or ionic strength.
pH working range 4–9
CIP stability 4–9
Storage 4 to 30°C, 20% Ethanol
Sanitization Wash the column with 0.5 M NaOH
Pack size 1 L
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For Research or Industrial Raw Materials, Not For Personal Medical Use!
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