| Cat# | Product Name | Size / Price | Qty | Inquiry |
|---|---|---|---|---|
| Se-497C | Sepharose 4B | Inquiry |
| Product Information | |
| Cat#No# | Se-497C |
| Product Overview | Sepharose 4B is a well-proven agarose size exclusion chromatography base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. |
| Description | Sepharose is a spherical agarose-based size exclusion chromatography matrix. Sepharose is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose 2B, Sepharose 4B and Sepharose 6B respectively, Both Sepharose and Sepharose CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight. |
| Characteristic | 4% Agarose size exclusion chromatography resin. Proven base matrix for coupling affinity ligands. |
| Maximum operating pressure | 70-140 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d. |
| Matrix | agarose, 4%, spherical |
| Particle Size | 45 μm-165 μm |
| Recommended flow rate | < 11.5 cm/h |
| Recommended column height | 10 cm |
| Chemical stability | Stable to commonly used aqueous buffers. |
| Physical stability | Negligible volume variation due to changes in pH or ionic strength. |
| pH working range | 4–9 |
| CIP stability | 4–9 |
| Storage | 4 to 30°C, 20% Ethanol |
| Sanitization | Wash the column with 0.5 M NaOH |
| Pack size | 1 L |
Download Datasheet
|
Download SDS
|
For Research or Industrial Raw Materials, Not For Personal Medical Use!Online Inquiry
