Cat# | Product Name | Size | Price | Qty | Inquiry |
---|---|---|---|---|---|
Se-497C | Sepharose 4B | Inquiry |
Product Information | |
Cat#No# | Se-497C |
Product Overview | Sepharose 4B is a well-proven agarose size exclusion chromatography base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. |
Description | Sepharose is a spherical agarose-based size exclusion chromatography matrix. Sepharose is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose 2B, Sepharose 4B and Sepharose 6B respectively, Both Sepharose and Sepharose CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight. |
Characteristic | 4% Agarose size exclusion chromatography resin. Proven base matrix for coupling affinity ligands. |
Maximum operating pressure | 70-140 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d. |
Matrix | agarose, 4%, spherical |
Particle Size | 45 μm-165 μm |
Recommended flow rate | < 11.5 cm/h |
Recommended column height | 10 cm |
Chemical stability | Stable to commonly used aqueous buffers. |
Physical stability | Negligible volume variation due to changes in pH or ionic strength. |
pH working range | 4–9 |
CIP stability | 4–9 |
Storage | 4 to 30°C, 20% Ethanol |
Sanitization | Wash the column with 0.5 M NaOH |
Pack size | 1 L |
Download Datasheet |
For Research or Industrial Raw Materials, Not For Personal Medical Use!
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