Sepharose CL-4B

• Cat#No#: Se-501C
• Product Overview: Sepharose CL-4B is a well-proven cross-linked agarose size exclusion chromatography base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
• Description: Sepharose is a spherical agarose-basedsize exclusion chromatography matrix. Sepharose is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose 2B, Sepharose 4B and Sepharose 6B respectively, Both Sepharose and Sepharose CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight.
• Characteristic: 4% cross-linked agarose size exclusion chromatography resins.
  Proven base matrix for coupling affinity ligands.
  Higher degree of crosslinking improve matrix stability.
• Maximum operating pressure: 80-150 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d.
• Matrix: cross-linked agarose, 4%, spherical
• Recommended flow rate: < 26 cm/h
• Recommended column height: 10 cm
• pH working range: 3–13
• CIP stability: 2–14
• Autoclavable: Autoclavable, 20 min at 120°C in pH 7.
• Storage: 4 to 30°C, 20% Ethanol
• Sanitization: Wash the column with 0.5 M NaOH
• Pack size: 1 L