SOURCE 15Q

• Cat#No#: SO-458C
• Product Overview: Source 15Q is a polymeric, strong anion exchanger designed for polishing steps in both research and industrial applications.
• Description: SOURCE 15Q is a synthetic high performance, preparative, chromatography resin, based on a 15 µm monosized, rigid polystyrene/divinyl benzene polymer matrix. It is modified with quaternary ammonium (Q) strong anion exchange groups. SOURCE resins have excellent physical and chemical characteristics, allowing high flow rates and consistent performance.
• Characteristic: Mono-sized 15 µm rigid beads gives low back pressure and high flow rates allowing for high productivity.
  Strong anion exchanger designed for high resolution polishing purification of proteins, peptides and oligonucleotides.
  High chemical stability allowing for wide range of working conditions with good resistance to cleaning conditions at high pH.
• Maximum operating pressure: 400 cm/h, 1000 kPa, FineLine 100 column.
• Ligand Coupling Method: long, hydrophilic spacer arms
• Packing Column: 1. Pour the resin slurry into the column in one continuous motion. Pouring down a glass rod held against the wall of the column helps prevent the introduction of air bubbles. Immediately fill the remainder of the column and reservoir with 20% ethanol. Attach the lid on the packing reservoir and connect it to the pump.
  2. Open the column outlet and start the packing by pumping 20% ethanol through the column at a flow velocity of approximately 1900 cm/ h for Tricorn 10/100. Switch off and disconnect the pump. Close the column outlet.
  3. Take the column from the stand and remove the packing reservoir over a sink. Remount the column vertically and fill to the top with 20% ethanol (20% ethanol with 0.2 M NaAc for SOURCE 15S).
  4. Wet the column adapter by submerging the plunger end in 20% ethanol and drawing through with a syringe. Make sure that all bubbles have been removed. Insert the adapter into the top of the column, taking care not to trap air under the net.
  5. With the adapter outlet open, push the adapter into the column and down approximately 2 mm into the resin bed, allowing the ethanol to displace any air remaining in the tubing.
  6. Lock the adapter in position, connect it to the pump, open the column outlet and continue packing for a further 15 min. Reposition the adapter on the resin surface if necessary.
• Matrix: Polystyrene/divinylbenzene
• Ionic Exchanger Type: Strong anion exchanger
• Particle Size: 15 µm-15 µm
• Average particle size: ~15 µm
• Ligand: Quaternary ammonium
• Dynamic binding capacity: ~ 45 mg BSA/mL resin
• Recommended flow rate: 150 to 900 cm/h
• Recommended column height: 10 cm
• Chemical stability: Stable in common ion exchange buffers.
• pH working range: 2–12
• CIP stability: 1–14
• Temperature stability: 4˚C to 40˚C
• Autoclavable: 20 min at 121°C in H2O, pH 7, 1 cycle.
• Storage: 4 to 30°C, 20% Ethanol
• Shipping: 20% ethanol
• Cleaning-in-place: Remove ionically bound proteins by washing the column with 0.5 bed volumes of a 2 M NaCl solution at a flow velocity of approximately 90 cm/h, contact time 10 to15 minutes, reversed flow direction.
  Remove precipitated proteins, hydrophobically bound proteins and lipoproteins by washing the column with 1.0 M NaOH solution at a flow velocity of approximately 40 cm/h, contact time 1 to 2 hours, reversed flow direction.
  Remove strongly hydrophobically bound proteins, lipoproteins, and lipids by washing the column with four bed volumes of 70% ethanol or 30% isopropanol at 10 cm/h, reversed flow direction. Apply increasing gradients to avoid air bubble formation when using high concentrations of organic solvents. Alternatively, wash the column with two bed volumes of 0.1 to 0.5% nonionic detergent in a basic or acidic solution. After treatment with detergent always remove residual detergent by washing with five bed volumes of 70% ethanol.
• Sanitization: Sanitization reduces microbial contamination of the bed to a minimum. Wash the column with 0.5 to 1.0 M NaOH at a flow velocity of approximately 40 cm/h, contact time 30 to 60 minutes, reversed flow direction.
• Pack size: 10 mL
• BioProcess resin: Yes
• Maximum flow velocity: 1800 cm/h
• Dimensions: 4.6 × 100 mm
• Column volume: 1.7 mL
• Functional group: –CH2–O–CH2–CHOH–CH2–O–CH2– CHOH–CH2–N+ (CH3)3