SOURCE 30RPC

• Cat#No#: SO-473C
• Product Overview: Source 30RPC is designed for reversed phase chromatography (RPC) with full pH range stability and suitable for intermediate purification and polishing in industrial processes requiring high flow velocities and low back-pressure.
• Description: SOURCE 30RPC is an addition to the SOURCE product range, a family of high performance separation resins for fast, preparative purification of biomolecules such as proteins, peptides, and oligonucleotides (Fig 1). SOURCE 30RPC is designed for reversed phase chromatography (RPC) and is an alternative to silica-based RPC matrices. The resin is based on a monodisperse polymeric matrix, offering high capacity and wide pH stability.
  SOURCE 30RPC is an excellent chromatography resin for the polishing stage of industrial purification processes where high productivity and stable performance in production are important.
• Characteristic: Mono-sized 30 µm rigid beads gives low back pressures and high flow rates allowing for high productivity.
  Designed for high resolution reversed phase chromatography of proteins, peptides and oligonucleotides.
  High chemical stability allowing for wide range of working conditions with good resistance to cleaning conditions at high pH. BioProcess resin supported for industrial applications.
• Maximum operating pressure: 2000 cm/h, 1000 kPa, FineLINE 100 column, bed height 10 cm, i.d. 10 cm
• Matrix: Polystyrene/divinyl benzene
• Average particle size: ~30 μm monosized
• Ligand: Matrix surface
• Dynamic binding capacity: ~14 mg BSA/mL resin at 300 cm/h, ~23 mg bacitracin/mL resin at 300 cm/h, ~72 mg insulin/mL resin at 300 cm/h.
• Recommended flow rate: 100 to 1000 cm/h
• Recommended column height: 10 cm
• Chemical stability: Stable in commonly used aqueous buffers 1.0 M HCl, 1 M NaOH, 1 M HCl/ 90% methanol, 90% HAc, 0.45 M NaOH/40% 2-propanol, 6 M guanidine hydrochloride, n-propanol, 20% ethanol, acetone.
• pH working range: 1–12
• CIP stability: 1–14
• Temperature stability: 4°C to 40°C
• Autoclavable: 20 min at 121°C
• Storage: 4 to 30°C, 20% Ethanol or 70% Acetonitrile
• Shipping: 20% ethanol
• Pack size: 200 mL
• BioProcess resin: Yes