SP Sepharose Big Beads

• Cat#No#: SP-461C
• Product Overview: SP Sepharose Big Beads is a strong cation exchanger designed for industrial applications in the capture step of large volumes of feed or viscous feedstocks.
• Description: SP Sepharose Big Beads is composed of large crosslinked agarose beads (100-300 µm) modified with sulphonate (SP) strong cation exchange groups. It is designed for industrial applications and the large particle size and physical stability of the base matrix ensure maintained performance and low back-pressures even with viscous samples.
• Characteristic: Easy to scale-up.
  High flow rates.
  High chemical resistance for effective cleaning-in-place (CIP).
  Easy maintenance.
• Maximum operating pressure: 1200-1800 cm/h, 100 kPa, XK 50/60 column
• Ligand Coupling Method: Ether bonds
• Metal ion capacity: 0.18-0.25 mmol H+/ml medium
• Packing Column: SP Sepharose Big Beads is easy to pack in small and large scale columns. Narrow peaks with high symmetry are reproducible whether you pack the ion exchanger bed with a constant pressure of between 1 to 3 bar, or let the slurry sediment and then compress it with the adaptor. Suction packing can easily be performed as well.
• Matrix: 6% cross-linked agarose
• Ionic Exchanger Type: Strong cation exchanger
• Particle Size: 100 µm-300 µm
• Average particle size: ~200 µm
• Ligand: Sulphopropyl
• Recommended flow rate: 1200 to 1800 cm/h
• Recommended column height: 25 cm
• Chemical stability: Stable in commonly used aqeous buffers, 1M NaOH, 70% ethanol, organic solvents.
• pH working range: 4–12
• CIP stability: 3–14
• Temperature stability: 4 to 30˚C
• Storage: 4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate.
• Cleaning-in-place: Cleaning-in-place Ionically bound proteins: Wash with filtered 2 M NaCl at approximately 100 cm/h. Contact time: 10 to 15 min.
  Hydrophobically bound proteins or lipoproteins: Wash with 1.0 M NaOH at 40 cm/h. Contact time: 1–2 h.
  Lipids and very hydrophobic proteins: Wash with 70% ethanol at 40 cm/h, reversed flow, or with saw-tooth gradient 0–30–0% isopropanol. Contact time: 1–2 h.
• Sanitization: A reduction of microbial contamination in the ion exchanger bed is obtained by washing the column with 0.5–1.0 M NaOH, allowing a contact time of 30–60 min.
• Pack size: 1 L
• BioProcess resin: Yes