SP Sepharose Fast Flow resin

• Cat#No#: SP-462C
• Product Overview: SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification.
• Description: The popularity of Sepharose Fast Flow ion exchange (IEX) chromatography resins reflects the important role they play in protein purification today. The reliability and well-documented performance of the resins have made them a common choice for capture and intermediate purification of proteins in both research and industry
• Characteristic: Well established strong cation exchanger.
  Based on Sepharose Fast Flow ion exchange resin.
  High chemical stability enables proven CIP and sanitization protocols.
  Hydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool. Fulfills industrial demands for security of supply, robust performance, and regulatory support.
• Maximum operating pressure: 400-700 cm/h, 100 kPa, XK 50/30 column
• Metal ion capacity: 0.18-0.25 mmol H+/ml medium
• Matrix: cross-linked agarose, 6%, spherical
• Ionic Exchanger Type: Strong cation exchanger
• Particle Size: 45 µm-165 µm
• Average particle size: ~90 µm
• Ligand: Sulphopropyl
• Dynamic binding capacity: ~ 70 mg ribonuclease A/mL resin
• Recommended column height: 15 cm
• Chemical stability: Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol.
• pH working range: 4–13
• CIP stability: 3–14
• Temperature stability: 4°C to 40°C
• Storage: 4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate.
• Cleaning-in-place: Ionically bound proteins: Wash with 0.5 CV filtered 2 M NaCl solution. Contact time 10 to 15 minutes. Reversed flow direction.
  Precipitated, hydrophobically bound proteins or lipoproteins: Wash with 1.0 M NaOH at 40 cm/h. Contact time 1 to 2 hours.
  Lipids and very hydrophobic proteins: Wash with 2 to 4 CV 0.5% non-ionic detergent (for example, 1 M acetic acid). Contact time 1 to 2 hours. Reversed flow direction. or, wash with 2 to 4 column volumes of up to 70% ethanol or 30% isopropanol. Contact time 1 to 2 hours. Reversed flow direction.
• Sanitization: To reduce microbial contamination in the packed column, sanitization using 0.5 to 1.0 M NaOH with a contact time of 1 hour is recommended.
• Pack size: 300 mL
• BioProcess resin: Yes
• Functional group: -CH2 -CH2 -CH2 SO3 -