SP Sepharose High Performance

• Cat#No#: SP-463C
• Product Overview: SP Sepharose High Performance is a strong cation exchange BioProcess resin with high resolution designed for intermediate and polishing steps in downstream processing.
• Description: SP Sepharose High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with sulphopropyl (SP) strong cation exchange groups. It is designed for intermediate purification and polishing applications and to be used when resolution and capacity have priority. SP Sepharose High Performance has high chemical stability and withstands effective CIP/sanitization protocols using sodium hydroxide.
• Characteristic: Strong cation exchanger for intermediate and final polishing steps.
  High resolution, high capacity separation with high recovery.
  High chemical stability for effective CIP/sanitization.
  BioProcess resin supported for industrial applications and well-established in approved processes.
• Maximum operating pressure: 150 cm/h (at < 3 bar, 25°C, 10 cm bed height)
• Ligand Coupling Method: Ether linkages
• Metal ion capacity: 0.15-0.20 mmol H+/ml
• Matrix: 6% cross-linked agarose
• Ionic Exchanger Type: Strong cation exchanger
• Particle Size: 24 µm-44 µm
• Average particle size: ~34 µm
• Ligand: Sulphopropyl
• Dynamic binding capacity: ~ 55 ribonuclease A/mL resin
• Recommended column height: 10 cm
• Chemical stability: Stable in commonly used buffers: 8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH,* 1 M acetic acid, 30% isopropanol, 30% acetonitrile, 2% SDS.
• pH working range: 4–13
• CIP stability: 3–14
• Storage: 4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate.
• Cleaning-in-place: Remove ionically bound proteins by washing the column with 0.5 bed volumes of a 2 M NaCl solution, contact time 10 to 15 minutes, reversed flow direction.
  Remove precipitated proteins, hydrophobically bound proteins and lipoproteins by washing the column with 1 M NaOH solution at a linear flow rate of approximately 40 cm/h, contact time 1 to 2 hours, reversed flow direction.
  Remove strongly hydrophobically bound proteins, lipoproteins and lipids by washing the column with four bed volumes of 70% ethanol or 30% isopropanol at 10 cm/h, reversed flow direction. Apply increasing gradients to avoid air bubble formation when using high concentrations of organic solvents.
• Sanitization: Wash the column with 0.5 to 1 M NaOH at a flow rate of approximately 40 cm/h, contact time 30 to 60 minutes, reversed flow direction.
• Pack size: 75 mL
• BioProcess resin: Yes
• Maximum flow velocity: 90 cm/h
• Functional group: -CH2 CH2 CH2 SO3 - , sulphopropyl (SP)