SP Sepharose XL

• Cat#No#: SP-464C
• Product Overview: SP Sepharose XL is a strong cation exchanger designed for use in the capture steps of biomolecules from clarified feed-stocks.
• Description: SP Sepharose XL is based on a highly cross-linked, bead formed 6% agarose matrix similar to the well established SP Sepharose Fast Flow resin. Dextran chains are covalently coupled to the agarose matrix and modified with sulphopropyl (SP) strong cation exchange groups through chemically stable ether bonds. The stable agarose matrix and long, flexible chains of dextran with bound charged groups work together to increase loading capacity whilst allowing high flow rates.
• Characteristic: High loading capacities give more productive capture from clarified feedstocks.
  Strong cation exchanger designed for capture steps.
  High chemical stability for effective CIP/sanitization.
  The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.
• Maximum operating pressure: 300-500 cm/h
• Ligand Coupling Method: Dextran extenders through ether bonds.
• Metal ion capacity: 0.18-0.25 mmol H+/ml medium
• Matrix: 6% cross-linked agarose with dextran surface extenders.
• Ionic Exchanger Type: Strong cation exchanger
• Particle Size: 45 μm-165 μm
• Average particle size: ~90 μm
• Ligand: Sulphopropyl
• Dynamic binding capacity: ≥ 160 mg lysozyme/mL resin
• Recommended flow rate: 200 cm/h
• Chemical stability: Commonly used buffers, nonionic detergents, 1 M NaOH, 6 M guanidine hydrochloride.
• pH working range: 4–13
• CIP stability: 3–14
• Temperature stability: 4˚C to 30˚C
• Storage: 4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate.
• Pack size: 300 mL
• BioProcess resin: Yes