Spdtran-25 Medium

• Cat#No#: SE-001S
• Product Overview: Spdtran is a beaded gel filtration medium prepared by crosslinking dextran and it is supplied in its dry form. Spdtran-25 can be used for protein and nucleic acid purifications being the exclusion limit 5 kD for proteins and 10 bases for nucleic acids. Desalting (before IEX and after HIC or Affinity Chromatography) & buffer exchange (between different chromatography steps) are other uses.
  Medium grade is suitable for separations at high flow rates and low operating pressures.
• Form: Dry form
• Matrix: Crosslinked dextran
• Water Regain: 2.15-2.25 ml/g
• Swelling: 4-6 ml/g
• Fractionation Range: 1-5 kD for globular proteins, 100-5,000 Da for dextrans
• Average particle size: Dry Particle Size 50-150 µm (>80%)
  Wet Particle Size 85-260 µm
• Maximum Pressure Drop: Maximum Operating Pressure: Generally obeys Darcy's Law: U=Ko ΔP/L Where: U= linear flow rate(cm/h). ΔP= pressure drop over gel bead (cm H2O). L= bed high(cm). Ko=80
• Chemical stability: All commonly used buffers, including: 0.1 M NaOH; 0.01 M HCl; 1 M acetic acid; 8 M urea; 6 M guanidine HCl; 1% SDS, 24% Ethanol; 30% Propanol; 30% Acetonitrile
• pH working range: pH Stability: 2.0 to 13
• Autoclavable: 121˚C, pH 7.0 (30 minutes)
• Storage: Ambient