Spdtran-50 Medium

• Cat#No#: SE-004S
• Product Overview: Spdtran is a beaded gel filtration medium prepared by crosslinking dextran and it is supplied in its dry form. Spdtran-50 can be used for protein and nucleic acid purifications being the exclusion limit 25 kD for proteins and 20 bases for nucleic acids.
  Medium grade is suitable for separations at high flow rates and low operating pressures.
• Form: Dry form
• Matrix: Crosslinked dextran
• Water Regain: 4.80-5.20 ml/g
• Swelling: 9-11 ml/g
• Fractionation Range: 1.5-30 kDa for globular proteins, 0.5-10 kDa for dextrans
• Average particle size: Dry Particle Size 50-150 µm (>80%)
  Wet Particle Size 100-300 µm
• Maximum Pressure Drop: Maximum Operating Pressure: Generally obeys Darcy’s Law: U=Ko ΔP/L Where: U= linear flow rate(cm/h). ΔP= pressure drop over gel bead (cm H2O). L= bed high(cm). Ko=145
• Chemical stability: All commonly used buffers, including: 0.1 M NaOH; 0.01 M HCl; 1 M acetic acid; 8 M urea; 6 M guanidine HCl; 1% SDS, 24% Ethanol; 30% Propanol; 30% Acetonitrile
• pH working range: pH Stability: 2.0 to 13
• Autoclavable: 121˚C, pH 7.0 (30 minutes)
• Storage: Ambient