Strong Anion Exchange Resin (Big Beads)

• Product Overview: IEC is a highly effective biomolecule separation method based on charge interactions, providing high binding capacity, resolution, controllable conditions, and scalability, widely used in pharmaceuticals, chemicals, metallurgy, and food. The medium consists of (1) cross-linked porous, hydrophilic, chemically stable matrix; (2) functional groups determining the medium’s properties; (3) counter-ions reversible with functional groups. Big Beads (BB) media are coupled with various functional groups on large, highly cross-linked agarose particles. The large particle matrix tolerates poorly clarified samples, reduces clogging, and allows high flow rates, suitable for processing large volumes quickly. Big particle agarose is a strong anion exchanger, capturing biomolecules directly from feedstock, with fast flow rates suitable for large-scale production.
• Matrix: Highly cross-linked agarose
• Appearance: White paste, may separate upon standing
• Wet Particle Size Range: 100–300 μm
• Ligand: Quaternary ammonium
• Fractionation Range: ~4 × 10⁶ (globular protein)
• Coupling chemistry: Quaternary ammonium functionalization
• Chemical stability: Stable in common aqueous solutions: 1 M NaOH, 6 M guanidine HCl, 30% isopropanol, 70% ethanol
• Maximum operating pressure: 0.3 MPa
• Pack size: 25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L; 20 L; 40 L
• Storage: 2–30 °C in 20% ethanol or 2% benzyl alcohol
• Product features: Large particle size (100–300 μm) anion exchange resin; suitable for rapid, efficient processing of large-volume feed; tolerates high flow rates and easy scale-up; good chemical stability, can withstand various solvents for in-place cleaning; widely applied for fast capture of high-viscosity samples in biopharmaceuticals.