IEC is a highly effective biomolecule separation technique based on charge interactions. The medium consists of a cross-linked porous, hydrophilic matrix, functional groups, and counter-ions. XL Big Beads media couple linear dextran onto large, highly cross-linked agarose particles, then functional groups onto dextran, reducing steric hindrance and increasing binding capacity. The large particle matrix tolerates poorly clarified samples, resists clogging, and allows high flow rates for large-volume processing. Extra Large Beads is a strong anion exchanger suitable for large-scale biomolecule capture with high capacity and flow rate.
Matrix
Highly cross-linked agarose with dextran chains
Appearance
White paste, may separate upon standing
Wet Particle Size Range
100–300 μm
Ligand
Quaternary ammonium
Capacity
230–330 μmol Cl- / mL packed resin
Coupling chemistry
Quaternary ammonium with dextran extension
pH working range
2–12
pH CIP range
2–14
Chemical stability
Stable in common aqueous solutions: 1 M NaOH, 6 M guanidine HCl, 30% isopropanol, 70% ethanol
Maximum operating pressure
0.3 MPa
Maximum flow velocity
300–900 cm/h
Pack size
25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L; 20 L; 40 L
Storage
2–30 °C in 20% ethanol or 2% benzyl alcohol
Product features
High-capacity, large particle size (100–300 μm) anion exchange resin; rapid, efficient processing of large-volume, high-viscosity feed; stable physical and chemical properties with long service life; widely applied for rapid rough purification of large-scale high-viscosity samples in biopharmaceutical and bioengineering downstream processes.
Download Datasheet
For Research or Industrial Raw Materials, Not For Personal Medical Use!