IEC separates biomolecules based on charge interactions. Highly cross-linked agarose (high performance) couples quaternary ammonium groups onto high-resolution agarose microspheres, forming a strong anion exchanger.
Matrix
Highly cross-linked agarose
Appearance
White paste, may separate upon standing
Average particle size
34 μm
Wet Particle Size Range
24–44 μm
Ligand
Quaternary ammonium
Dynamic binding capacity
~75 mg BSA / mL packed resin
Coupling chemistry
Quaternary ammonium functionalization
pH working range
2–12
pH CIP range
2–14
Chemical stability
Stable in common aqueous solutions: 0.5 M NaOH, 2 M NaOH, 0.1 M HCl, 70% formic acid, 6 M guanidine HCl, 8 M urea, 30% acetonitrile, 30% isopropanol, 24% ethanol, 70% ethanol, 50 mMPB + 2 M NaCl pH 7.0
Maximum operating pressure
0.3 MPa
Pack size
25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L; 20 L; 40 L
Storage
2–30 °C in 20% ethanol or 2% benzyl alcohol
Product features
High-resolution strong anion exchange resin; average particle size 34 μm highly cross-linked agarose matrix; high resolution and low non-specific adsorption; suitable for medium or fine purification of proteins, nucleic acids, and peptides in biopharmaceutical R&D and production.