Big Beads (BB) series media are large, highly cross-linked agarose particles, tolerating poorly clarified samples, resisting clogging, and allowing high flow rates. SP large particle agarose is a strong cation exchanger suitable for large-scale biomolecule capture with high capacity and flow rate.
Matrix
6% Highly cross-linked agarose
Appearance
White paste, may separate upon standing
Wet Particle Size Range
100–300 μm
Ligand
Sulfopropyl (SP)
Coupling chemistry
Sulfopropyl functionalization
pH working range
4–13
pH CIP range
3–14
Chemical stability
Stable in common aqueous solutions: 1 M NaOH, 6 M guanidine HCl, 30% isopropanol, 70% ethanol
Maximum operating pressure
0.3 MPa
Maximum flow velocity
≥ 1200 cm/h
Pack size
25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L; 20 L; 40 L
Product features
Large particle size (100–300 μm) cation exchange resin; suitable for rapid, efficient processing of large-volume feed; tolerates high flow rates and easy scale-up; good chemical stability, withstands various solvents for in-place cleaning; widely applied for crude purification of high-viscosity samples in biopharmaceuticals and protein extraction from milk.