Super Large Pore Size 700nm Q PMMA Resin

• productkeywords (多个词 半角分号";"分隔): Q; 700nm; LPS; Large Pore Size; 70X Q Supermacrous Ion Exchange Chromatography Media; LPS-70X-Q; Super Large Pore Size 700nm Q PMMA Resin; strong anion exchange chromatography
• Product Overview: 70X Q super large pore strong anion exchange chromatography medium is specially designed for the purification of viruses with a size of about 100nm or larger. It uses hydrophilic polymethyl methacrylate (PMMA) as the matrix, with a particle size of 70μm. Its unique 7000Å ultra-large pore design breaks the limitations of conventional chromatography media in purification. The extremely large pore diameter minimizes the impact on the structure and activity of the virus during the purification process, ensuring that the binding capacity does not decrease after multiple uses and the service life is longer. By means of the unique surface modification technology, ionic exchange functional groups are bonded to the hydrophilic matrix surface, thereby preparing a strong anion exchange (Q) chromatography medium.
• Ion Exchange Type: Strong Anion Exchange
• Product characteristics: 700nm super-large pore diameter.
• Advantage: High protein activity: With a super-large pore diameter of over 700nm, it can significantly reduce the degree of protein denaturation, thereby enhancing protein activity; High flow rate and strong anti-contamination ability: High mechanical strength, extremely large pore diameter, large virus particles are less likely to clog, and can maintain a higher loading capacity under high flow rate conditions; High stability and high recovery rate: The extremely large pore size significantly enhances the thermal stability of viruses during adsorption on the chromatographic medium, reduces virus lysis, and increases the yield of live viruses by more than 20%; Low non-specific adsorption: The surface of the microspheres is modified with hydrophilic treatment, which can solve the problem of strong adsorption caused by certain highly hydrophobic proteins or viruses.
• Material: PMMA
• Particle size: 70μm
• Pore Size: 700nm
• Chemical stability: All common buffers, 1M acetic acid, 1M sodium hydroxide, 1M hydrochloric acid, 70% ethanol, 30% isopropanol, 30% acetonitrile, 1% SDS, 6M guanidine hydrochloride, 8M urea and other common organic solvents; avoid contact with strong oxidizers.
• Working temperature: 4~30°C
• Per milliliter dosage: > 40mg BSA/mL
• Ligand density: 0.03 meq/mL
• Recommended flow rate: 150~600cm/h
• pH: 2~12(work) 1~14(CIP)
• Matrix: PMMA
• Ligand: -N+(CH 3)3
• Maximum Pressure: 1 Mpa
• Applications: Separation and purification of ultra-large biological particles such as viruses up to 100nm in size and VLPs.
• Storage Buffer: 20% ethanol
• Storage: Store at 2-30°C in 20% ethanol (long-term preservation is more favorable at 4°C); the medium in the chromatography column can be rinsed with 20% ethanol and then stored at 2-30°C.