Superdex 200 Prep Grade

• Cat#No#: Su-503C
• Product Overview: Superdex 200 prep grade size exclusion chromatography resin offers very high resolution fractionation for separation, polishing and product formulation of monoclonal antibodies.
• Description: Superdex prep grade is a preparative size exclusion chromatography resin with a composite matrix of dextran and agarose. This matrix combines the excellent size exclusion chromatography properties of cross-linked dextran (Sephadex) with the physical and chemical stabilities of highly cross-linked agarose, to produce a separation resin with outstanding selectivity and high resolution. In addition, its low non-specific interaction permits high recovery of biological material. Together these properties make Superdex prep grade the first choice size exclusion chromatography resin for all applications from laboratory to process scale.
• Characteristic: Size exclusion chromatography resin providing exceptional resolution with short run times and good recovery.
  Superdex 200 prep grade is recommended for the purification of monoclonal antibodies.
  BioProcess resin supported for industrial applications and well-established in approved processes.
• Maximum operating pressure: 40 to 60 cm/h at <0.3 MPa in a BPG 200 column with 20 cm diameter and 83 cm bed height (at room temperature using buffers with the same viscosity as water).
• Matrix: Composite of cross-linked agarose and dextran
• Particle Size: 24-44 µm
• Average particle size: ~34 µm
• Recommended column height: 83 cm
• Chemical stability: Stable in commonly used aqueous buffers for size exclusion chromatography, 1.0 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile.
• pH working range: 3–12
• CIP stability: 1–14
• Autoclavable: 20 min at 121°C, 1 cycle.
• Storage: 4 to 30°C, 20% Ethanol
• Sanitization: wash the column with 0.5 M NaOH at a reversed flow velocity of 10 cm/h for an hour. Before applying the sample, remove any traces of NaOH by washing the column with at least two column volumes of buffer, or until pH is stable, at 20 cm/h with normal flow direction.
• Pack size: 150 mL
• BioProcess resin: Yes
• Dimensions: 20 cm