Superdex 30 prep grade (PG) in HiLoad column

• Cat#No#: Su-321P
• Product Overview: HiLoad Superdex 30 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography separating peptides.
• Characteristic: High-resolution separation – with short run times and good recovery.
  High capacity – at high flow rates.
  Prepacked – for convenient ready-to-go use and reproducible results.
  Scalable protein separation – easily scale-up separations to production levels.
  Integrated workflows – 1/16” fittings for convenient connection with ÄKTA protein purification systems.
• Maximum operating pressure: 3 bar [0.3 MPa] (42 psi)
• Matrix: Cross-linked agarose, spherical
• Average particle size: ~ 34 μm
• Recommended flow rate: 10 cm/h - 50 cm/h
• Recommended column height: 600 mm
• pH working range: 3 to 12
• CIP stability: 1 to 14
• Storage: 4 to 30°C, 20% Ethanol
• Shipping: 20% ethanol
• Cleaning-in-place: Wash the column with one-half to one CV 0.5 M NaOH at a flow rate of 0.8 mL/min for 16/600 or 2.2 mL/min for 26/600 to remove most of the nonspecifically bound proteins from the chromatography resin. After cleaning, immediately equilibrate the column with at least two CV buffer. Further equilibration is necessary if the buffer contains detergents. Wait until the UV baseline stabilizes before starting a new purification.
• Dimensions: 16 × 600 mm
• Column volume: 120 mL
• Column i.d.: 16 mm
• Column hardware pressure limit: 0.5 MPa (5 bar, 73 psi)