Superose 6 Increase columns

• Cat#No#: Su-323P
• Product Overview: Superose 6 Increase SEC columns are designed for high-resolution, small-scale preparative purification, and analysis of large globular proteins, protein complexes, and other macromolecules in the molecular weight range Mr~ 5K to ~ 5M.
• Description: Superose 6 Increase prepacked columns are designed for rapid separation and analysis of proteins and other biomolecules by size exclusion chromatography (SEC, also known as gel filtration), in the molecular weight (Mr ) range for globular proteins between 5000 and 5 000 000. This very wide fractionation range of the chromatographic resin makes it suitable for purification of protein complexes, membrane proteins, and other macromolecules. The columns are also useful as a screening tool to explore the molecular-weight distribution of unknown samples. This new generation SEC resin replaces the well-known Superose 6 columns.
• Characteristic: Wide fractionation range: optimized to purify large biomolecules, including membrane proteins, protein complexes, and DNA fragments. Very high resolution: small bead size and a narrow particle size distribution provide high resolution, for high protein purity.
  High flow rates: rigid beads give excellent pressure/flow properties.
  Enhanced performance: improved resolution and runtime compared to predecessor.
• Matrix: Composite of cross-linked agarose.
• Average particle size: ~ 8.6 µm
• Recommended column height: 300 to 310 mm
• Chemical stability: Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.
• pH working range: 3 to 12
• CIP stability: 1 to 14
• Storage: 4˚C to 30˚C, 20% ethanol
• Maximum flow velocity: 1.50 mL/min, water at room temperature; 0.75 mL/min, 20% ethanol at room temperature; 0.75 mL/min water at low temperature; 0.35 mL/min 20% ethanol at low temperature.
• Dimensions: 10 × 300 mm
• Column volume: ~ 24 mL
• Column i.d.: 10 mm
• Bed support: Filter
• Material (bed support): Polyethylene (PE) filter
• Material (column tube): Borosilicate glass
• Material (column hardware): Borosilicate glass, polyetheretherketone (PEEK), polypropylene (PP), ethylene propylene diene monomer (EPDM), and perfluoro-rubber (PFR).