Superose 6 Prep Grade

• Cat#No#: Su-507C
• Product Overview: Good resolution separations of proteins and other biomolecules according to size, Wide separation range – separations in a molecular weight range from 5000 up to 5 million. Excellent reproducibility and durability.
• Description: Superose 6 is designed for high-resolution chromatography. Superose 6 Prep Grade permits easy, efficient scale-up to preparative separations.
• Characteristic: Achieve high-resolution separations across exceptionally broad molecular-weight ranges.
  Low ionic strength eluents (< 0.05 M) can utilize a hydrophobic interaction component to alter the selectivity of Superose for some lipids, peptides, and small aromatic compounds.
  High performance gel filtration resin specially designed for preparative purification of biomolecules.
• Sample preparation: Degas and filter all solutions through a 0.22 μm filter. Water should be distilled water quality. Use analytical grade solvents, salts, and buffers. Centrifuge (10 000 × G for 10 minutes) or filter samples through a 0.22 μm filter. Be sure to select a solvent resistant filter if samples are dissolved in organic solvents. If your sample is of high viscosity dilute it with the eluent.
• Matrix: Highly cross-linked agarose
• Particle Size: 30 µm-40 µm
• Recommended flow rate: up to 40 cm/h
• pH working range: 3–12
• CIP stability: 1–14
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: 1. Change the filter on the top of the medium bed (HR 16/50) following the instructions supplied with the Filter Kit HR 16 (see "Spare parts and accessories").
  2. Wash with 0.1 M-0.2 M NaOH: Turn the column up side down, inject at least 1/3 of the column volume and then elute with distilled water.
  3. Equilibrate before your next run, check pH, and turn the column back to normal position.
• Pack size: 125 mL
• Maximum operating backpressure: 0.4 MPa, 4 bar, 60 psi