Tagged package, His

• Cat#No#: Tag-120P
• Product Overview: His package enables convenient purification of histidine-tagged proteins.
• Description: Histidine tags are widely used because they are small and rarely interfere with the function, activity, or structure of target proteins. Immobilized metal ion affinity chromatography (IMAC) is the most common method for purifying histidine-tagged proteins. IMAC media, charged with divalent metal ions, such as nickel (Ni2+) or cobalt (Co2+), selectively retain histidine-tagged proteins and also allow for the purification of insoluble histidine-tagged proteins from inclusion bodies when denaturing conditions are used. Successful IMAC purification gives a high yield of pure and active target protein.
• Pack size: 1
• Kit contents: HisTrap HP (1 × 1 mL) HiTrap TALON crude (1 × 1 mL) HiTrap Desalting (1 × 5 mL)