TALON Superflow resin

• Cat#No#: TA-407C
• Product Overview: TALON Superflow is a cobalt-based IMAC resin that offers a high-specificity alternative to nickel-charged resins for purifying histidine-tagged proteins.
  High specificity – designed for histidine-tagged (his-tagged) purification of recombinant proteins when purity is more important than yield.
  Different selectivity compared to nickel-charged resins – an alternative to nickel affinity resins when a different metal ion is required.
• Description: TALON Superflow is a cobalt-based immobilized metal affinity chromatography medium (IMAC) offering enhanced selectivity for histidine-tagged proteins compared to nickel-charged media. TALON Superflow is available in 10 ml and 50 ml lab packs, and prepacked in a range of columns and 96-well plates. Prepacked HiTrap TALON crude, His GraviTrap TALON, His SpinTrap TALON, and His MultiTrap TALON enable different throughput and scales from screening in low microgram scale to milligram preparative purification of histidine-tagged recombinant proteins by IMAC.
• Characteristic: Suitable for IMAC screening and purification of histidine-tagged proteins when Ni2+ is not the optimal choice of metal ion.
  Compatible with commonly used IMAC reagents and appropriate for purifying proteins under native or denaturing conditions.
  HiTrap TALON crude and His GraviTrap TALON save time by allowing the application of unclarified samples directly, minimizing labor and degradation of proteins.
  His SpinTrap TALON and His MultiTrap TALON enable screening with highly reproducible results column-to-column and well-to-well.
  High purity in one step using a mild purification process that preserves structure and function of sensitive histidine-tagged proteins.
• Packing Column: TALON Superflow is supplied preswollen in 20% ethanol. Prepare a slurry by decanting the 20% ethanol solution and replacing it with distilled water in a ratio of 75% settled medium to 25% distilled water.
• Matrix: Cross-linked agarose, 6%
• Particle Size: 60 μm-160 μm
• Ligand: TALON Superflow Cobalt-IMAC
• Dynamic binding capacity: Up to 20 mg histidine-tagged protein
• Chemical compatibility: Stable in all commonly used buffers, detanurants, and detergents.
• pH working range: 3–12
• CIP stability: 2–14
• Storage: 4°C to 8°C
• Pack size: 10 mL
• Maximum flow velocity: 2000 cm/h
• Wash buffer: 50 mM sodium phosphate, 300 mM NaCl, 5 mM imidazole, pH 7.4.