Thiopropyl Sepharose 6B

• Cat#No#: Th-384C
• Product Overview: Thiopropyl Sepharose 6B is a resin used for reversible immobilization of molecules containing thiol groups under mild conditions. Optimized for immobilization of small biomolecules:
  Reversible coupling of proteins and small thiolated ligands through thiol groups to Sepharose 6B via a small hydroxypropyl spacer arm. Contains reactive 2-thiopyridyl disulphide groups attached to Sepharose through a chemically stable ether linkage.
  For immobilizing low molecular weight substances.
  Separate thiol-containing proteins and peptides from non-thiol-containing proteins and peptides.
• Description: Thiopropyl Sepharose 6B contains reactive 2-thiopyridyl disulphide groups attached to Sepharose through a chemically stable ether linkage. Thiopropyl Sepharose 6B reacts with solutes containing thiol groups under mild conditions to form mixed disulphides. This reaction forms the basis of covalent chromatography and a procedure for reversible immobilization of thiol containing molecules.
• Matrix: Agarose, 6%
• Particle Size: 45 µm-165 µm
• Average particle size: ~90 µm
• Ligand density: 20 µmol activated thiol/ml medium
• Chemical stability: Stable to commonly used aqueous buffers and additives like detergents. Azides should be avoided.
• pH working range: 2–8
• CIP stability: 2–8
• Storage: 2 to 8°C
• Pack size: 15 g
• Group To be Coupled: -SH