Ion exchange chromatography (IEC) is a highly effective method for biomolecule separation and purification. It relies on interactions between positive and negative charges, separating biomolecules based on their charge properties and quantities under specific conditions. IEC offers high loading capacity, good resolution, controllable conditions, and scalability, and is widely used in pharmaceuticals, chemicals, metallurgy, and food industries. Ion exchange media consist of three components: (1) a crosslinked porous, hydrophilic, and chemically stable matrix; (2) functional groups attached to the matrix that determine the properties of the medium; (3) counter-ions (equilibrium ions) that reversibly bind to the functional groups. Carboxymethyl (CM) is a weak cation exchange medium formed by coupling carboxymethyl groups onto high-rigidity agarose beads, offering higher pressure tolerance and loading capacity than CM highly cross-linked agarose.
Matrix
High-rigidity agarose with dextran chains
Appearance
White paste, may separate upon standing
Wet Particle Size Range
45–165 μm
Ligand
Carboxymethyl (CM)
Dynamic binding capacity
~50 mg BSA / mL packed resin
Coupling chemistry
Carboxymethyl (CM) functionalization
pH working range
4–13
pH CIP range
2–14
Chemical stability
Stable in common aqueous solutions: 1 M NaOH, 1 M acetic acid, 6 M guanidine HCl, 8 M urea, 70% ethanol, 30% isopropanol, 20% ethanol, 2% benzyl alcohol
Maximum operating pressure
0.5 MPa
Maximum flow velocity
≥ 1500 cm/h
Pack size
25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L; 20 L; 40 L
Product features
High-rigidity agarose matrix weak cation exchange resin; high rigidity, low back pressure, suitable for high flow processing of large-scale samples; good physical and chemical stability, low non-specific adsorption; widely applied for large-scale separation/purification of recombinant proteins in biopharmaceuticals and bioengineering.
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For Research or Industrial Raw Materials, Not For Personal Medical Use!