WSHPQFEK tag Protein Purification Kit

• Product Overview: WSHPQFEK-tag II is composed of only eight chemically balanced amino acid short peptides (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys), making the effect on the structure and activity of the recombinant protein negligible. Therefore, during the purification process, there is no need to remove WSHPQFEK-tag II from the target protein; Meanwhile, the target protein can be purified under physiological conditions. Compared with other Tags, these mild purification parameters can preserve the biological activity of the protein and obtain the target protein with a purity of over 99%.
• Particle size: 30-150 μm
• Binding capacity: ~7 mg WSHPQFEK-tag II protein/mL Gel
• Suspension concentration: 10%（v/v）magnetic beads suspension
• Elution Buffer: 2.5 mM desthiobiotin in Binding Buffer
• Regeneration Buffer: 0.5 MNaOH or 1 mM HABA
• Ligand: ~6 mg WSHPQFEKTactin /mL Gel
• Binding/Washing Buffer: 10 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH8.0
• Applications: It can be used for the isolation and purification of WSHPQFEK-Tag II tag proteins from any expression system, including baculoviruses, mammalian cells, yeast and bacteria.
• Storage Buffer: 1 x PBS(0.1% Tween-20+0.05% NaN3)
• Storage: 2-30°C(For long-term storage, it is recommended to keep at 2- 8°C).