Immobilized-Metal Affinity Chromatography (IMAC)

Introduction of Immobilized-Metal Affinity Chromatography (IMAC)

A commonly used protein purification technology, immobilized-metal ion affinity chromatography (IMAC), uses the interaction between metal ions and affinity groups (such as histidine, cysteine, etc.) in proteins to separate the target protein from the mixture. In the IMAC column, the commonly used metal ions are Ni²⁺, Cu²⁺, Zn²⁺, etc. These metal ions can form coordination bonds with the affinity groups in the protein so that they can interact with each other. Since this interaction is reversible, protein adsorption and elution can be controlled by varying conditions.

Fig. 1 Overview of an immobilized metal affinity chromatography (IMAC) experiment for metalloproteomic analysis. (Chang Y Y, et al., 2017)

The Superiority of His-Tag Purification

• It has strong binding force and large protein binding capacity, so it can be used in industrial production.
• The elution conditions are mild, and the ligand recovered completely after regeneration.
• By modifying various metal ions, the most suitable metal ions for the target protein can be introduced to purify different proteins.
• With good selectivity, IMAC can selectively adsorb the target protein.
• Due to the strong affinity, IMAC can utilize the interaction between metal ions and affinity groups in proteins to achieve efficient protein purification.
• The operation is simple and can be carried out under normal laboratory conditions.

Advantages of Our Products

• Mechanical stable and designed for scaling up His tag purification
• High yield
• Low leakage of nickel ions
• EDTA-resistant
• High selectivity for high purity
• Retained high 6xHis affinity in various buffers
• Recharge-free with minimal nickel ion leakage
• Requires less imidazole
• Versatile, ready-to-use, and convenient format

Applications of Our Products

• Routine His tag protein purification for structural biology and functional assays
• Scaling up and preparative applications
• Fractionation of protein fractions, proteomics, as well as immobilization and detection of proteins

Creative BioMart can provide you with a variety of high-quality IMAC purification products for the purification and separation of high-purity proteins, enzymes, amino acids, and peptides in a series of life science research. You can quickly find the reagents you need by searching keywords such as CAT number or product name. Please tell us your product needs, we will provide you with a comprehensive consultation. If you have any questions, please feel free to contact us.

Reference:

1. Chang Y Y, Li H, Sun H. (2017) Immobilized Metal Affinity Chromatography (IMAC) for Metalloproteomics and Phosphoproteomics. Inorganic and Organometallic Transition Metal Complexes with Biological Molecules and Living Cells. 329–353.