Creative BioMart Chromatography offers effective immobilization service to achieve robust coupling between ligand and resin matrix while maintaining ligand activity. Affinity chromatography is generally considered as the most effective method for substance separation. An important step in the preparation of affinity resins is to immobilization the purified ligands on supporting matrices. Exploring ligands immobilization conditions often require a lot of time and cost. Ligand Immobilization service requires the ligand to be covalently linked to an insoluble matrix. The types of functional groups commonly used for attachment include reactive components, such as primary amines, aldehydes, sulfhydryl groups, and carboxylic acids. In general, the solid matrix is activated by these functional groups, and then, a covalent bond is formed between the ligand and the support, resulting in ligand immobilization.
Schematic Examples of Ligand Immobilization
NHS Ester-activated Supports
NHS ester is a reactive group formed by EDC activation of carboxylate molecule. NHS ester-activated resins react with primary amines in alkalescency conditions (pH 7.2-8.5) to form stable amide bonds, which leads to immobilization of the ligands.
Fig. 1 Ligand immobilization on NHS-ester agarose resin.
Aldehyde-activated Supports
Reductive amination is a reliable and popular chemical process for covalently attaching antibodies or other proteins to beaded agarose resins. The reaction begins with the formation of an initial Schiff base between an aldehyde and an amine group, followed by the addition of NaCNBH3 to reduce to a secondary amine. Depending on the type and amounts of ligands involved, the coupling efficiency of the reaction can be more than 85%.
Fig. 2 Ligand immobilization on aldehyde-activated agarose resin.
| Ligand Type | Resin Matrix | Immobilization Method |
|---|---|---|
| Ligand | Agarose | Reductive Amination (reactive aldehyde) |
| Antigen | Silica | Streptavidin-Biotin |
| Antibody | PS-DVB | Extended Chain NHS |
| Enzyme | PMA | Extended Chain Epoxy |
| DNA/RNA | magnetic particles | Extended Chain CNBr |
| Other | nanoparticles | Customer selected Immobilization Method |
| microplates | ||
| Customer Provided Matrix |
Our Specialty
- Professional immobilization service, the coupling efficiency > 85%, reduce the use of expensive biomolecules such as antibody protein.
- U.S. Shirley, NY-based operation for efficient and reliable sample delivery.
- Provide test and verification reports and complete lab reports.
- Sign agreements to protect the intellectual property and information of our clients.
Creative BioMart Chromatography has established a mature ligand immobilization technology platform with years of technology accumulation. Relying on this technology platform, Creative BioMart Chromatography has developed a variety of popular coupling resins and coupling kits to provide customers with maximum support. Our customers have direct access to our experts and give timely feedback to any online inquiries. If you are interested in our services, please contact us.
Ligand Immobilization Service Process
*If your organization requires the signing of a confidentiality agreement, please contact us by email.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.